E-MEXP-1028 - Transcription profiling by array of CIC-2 knock out mice

Status
Released on 1 May 2008, last updated on 14 August 2014
Organism
Mus musculus
Samples (14)
Array (1)
Protocols (6)
Description
ClC-2 is a broadly expressed Cl- channel of the CLC family of Cl- channels and transporters which is abundantly expressed in brain. Here it was proposed to participate in lowering the cytoplasmic Cl- concentration of neurons, a process that establishes an inhibitory response to the neurotransmitters GABA and glycine (Staley et al., 1996). Heterozygous mutations in CLCN2 (the gene encoding ClC-2) were recently reported in a few patients with three clinically distinct forms of epilepsy (Haug et al, 2003). However, the disruption of ClC-2 in mice (ClC-2 KO mouse) did not entail epilepsy (Bösl et al., 2001; Nehrke et al., 2002) but myelin vacuolation in fiber tracts of the central nervous system. We used a gene expression profiling of the ClC-2 KO mouse in brain to identify possible disease mechanism which cause the observed myelin phenotype. As these myelin vacuolation became apparent in the fiber tracts of ClC-2 KO cerebellum at P28 and increased with age, we analysed the cerebellum of ClC-2 KO mice at different postnatal ages, before (P14) and after (P35) the KO cerebellum has been affected by myelin vacuolation.
Experiment types
transcription profiling by array, co-expression, development or differentiation, disease state, genetic modification
Contact
Citation
Leukoencephalopathy upon disruption of the chloride channel ClC-2. Judith Blanz, Christian A. Huebner, Michaela Schweizer, Hannes Maier, Adrian Muenscher, Thomas J. Jentsch. , Europe PMC 17567819
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MEXP-1028.idf.txt
Sample and data relationshipE-MEXP-1028.sdrf.txt
Raw data (1)E-MEXP-1028.raw.1.zip
Processed data (1)E-MEXP-1028.processed.1.zip
Additional data (1)E-MEXP-1028.additional.1.zip
Array designA-AFFY-23.adf.txt
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