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E-GEOD-9714 - Transcription profiling of human radio sensitive and radio resistant head neck tumor cell lines after xenograft into nude mices

Status
Submitted on 28 November 2007, released on 16 June 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (6)
Description
Nu61, a radiation-resistant human tumor xenograft, was selected from a parental radiosensitive tumor SCC-61 by eight serial cycles of passage in athymic nude mice and in vivo irradiation. Obtained tumors xenografts were profiled in two independent experiments using Affymetrix U133A arrays. Most abundant gene pattern associated with radioresistant phenotype was presented by IFN-inducible, Stat1-dependent pathway. In these experiments we detected genes responding to IFN alpha, beta and gamma in nu61 and SCC61 Experiment Overall Design: Cell lines derived from nu61 and SCC61 were grown in vitro and treated by the mixture of IFNalpha, IFNbeta and IFN gamma (100,100 and 5ng/ml respectively). 5 hours after administration of IFNs cells were lysed, RNA collected and used for hybridization with U133A chips to detect genes responding to IFN treatment in nu61 and SCC61
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-9714.idf.txt
Sample and data relationshipE-GEOD-9714.sdrf.txt
Raw data (1)E-GEOD-9714.raw.1.zip
Processed data (1)E-GEOD-9714.processed.1.zip
Array designA-AFFY-33.adf.txt
Links