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E-GEOD-9713 - Transcription profiling of human xenografted radioresistant and radiosensitive tumors before and after irradiation

Status
Submitted on 28 November 2007, released on 16 June 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (18)
Array (1)
Protocols (7)
Description
Nu61, a radiation-resistant human tumor xenograft, was selected from a parental radiosensitive tumor SCC-61 by eight serial cycles of passage in athymic nude mice and in vivo irradiation. Obtained tumors were profiled using Affymetrix U133A arrays. Most abundant gene pattern associated with radioresistant phenotype was presented by IFN-inducible, Stat1-dependent pathway Experiment Overall Design: Obtained tumors were established as xenografts in nude mice. RNA were purified from each xenograft, normalized by concentration and used for hybridization with each array. Three arrays were used for each group (un-treated nu61 and SCC61 and irradiated nu61 and SCC61 5 and 24 hours post-IR). Each group contained from 5 animals; tumors were randomized by size and three tumors from each group were used for profiling
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-9713.idf.txt
Sample and data relationshipE-GEOD-9713.sdrf.txt
Raw data (1)E-GEOD-9713.raw.1.zip
Processed data (1)E-GEOD-9713.processed.1.zip
Array designA-AFFY-33.adf.txt
Links