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E-GEOD-9450 - Transcription profiling of rice plants infected by rice blast fungus

Status
Submitted on 29 October 2007, released on 16 June 2008, last updated on 2 May 2014
Organism
Oryza sativa
Samples (72)
Array (1)
Protocols (19)
Description
To investigate plant-fungus interactions in early stage of infection, we analyzed response of rice against Magnaporthe grisea infection deficient mutants. In M. grisea, Mgb1 and Mst12 are essential for development of infection structures. Deletion of MGB1 results in defect in appresorium formation, and MST12, in penetration peg development. Analysis of gene expression profiles in rice by microarray revealed the mutant-specific and R gene dependent gene expression, strongly suggesting that gene-for-gene interaction commences before the penetration into rice cell. Experiment Overall Design: Agilent rice oligo microarray was used to investigate the gene expression profiling in rice plants infected with Guy11, Mgb1 and Mst12. Total RNAs were isolated from fourth leaves at 24 and 48 hpi. Agilent rice DNA chips were used according to the manufacture’s protocol. After hybridization, arrays were scanned using an Agilent Microarray Scanner, and Feature Extraction version 9.1 was used for image analysis and data extraction. Signal intensities derived from inoculated leaved were compared with that of mock-inoculated leaves. In each treatment, the experiment was performed independently three times.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-9450.idf.txt
Sample and data relationshipE-GEOD-9450.sdrf.txt
Processed data (1)E-GEOD-9450.processed.1.zip
Array designA-AGIL-10.adf.txt
Links