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E-GEOD-9182 - siRNA profiling of human LNCaP cells overexpressing/knockdown of HOXC6

Status
Submitted on 27 September 2007, released on 20 October 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (6)
Description
The transcription factor HOXC6 is upregulated in human prostate cancer. SiRNA knockdown of HOXC6 induces apoptosis in LNCaP cells while upregulation rescued LNCaP cells from siRNA-induced apoptosis. We used microarrays to identify the genes whose expression underly the anti-apoptotic and proliferative effects of HOXC6 in LNCaP cells. Experiment Overall Design: LNCaP cells were transiently transfected with a cocktail of 5 siRNAs to HOXC6 to a final concentration of 100nM, with a HOXC6 gene expression vector, or control siRNA and vector. Cells were collected a 48hours post-transfection
Experiment types
RNAi profiling by array, unknown experiment type
Contact
Colleen D. McCabe
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-9182.idf.txt
Sample and data relationshipE-GEOD-9182.sdrf.txt
Raw data (1)E-GEOD-9182.raw.1.zip
Processed data (1)E-GEOD-9182.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-9182.eSet.r
Links