E-GEOD-9066 - Transcription profiling of human HEK cells and hamster A23 cells transfected with Pcdh7 (a gene identified as a regulator of many genes) or empty vector to validate results of prediction of upregulated genes
Submitted on 17 September 2007, released on 3 April 2008, last updated on 2 May 2014
Cricetulus griseus, Homo sapiens
We have mapped regulatory loci for nearly all protein coding genes in the mammalian genome using microarray measurements from a panel of mouse/hamster radiation hybrids. Proof of principle transfection experiment. Pcdh7 (a gene identified as being a regulator of many genes) is transfected into A23 and HEK cells and compared to A23 and HEK cells transfected with an empty vector. Upregulated genes predicted by our approach are compared with the transfection experiments Experiment Overall Design: There are 4 microarrays. The first two are biological replicates of A23 (transfected with Pcdh7, labeled with cy5) / A23 (tranfected with empty vector, labeled with cy3). The second two are biological replicates of HEK (transfected with Pcdh7, labeled with cy5) / HEK (tranfected with empty vector, labeled with cy3).
transcription profiling by array, unknown experiment type