E-GEOD-8974 - Transcription profiling of pig coronary arterial endothelial cells by multiple passaging in vitro

Status
Submitted on 6 September 2007, released on 16 June 2008, last updated on 2 May 2014
Organism
Sus scrofa
Samples (8)
Array (1)
Protocols (7)
Description
Nitric oxide helps to prevent endothelial dysfunction and senescence. This study aimed to define genomic and proteomic changes in cultured porcine senescent endothelial cells and their resemblance with those observed in regenerated endothelial cells. Senescent endothelial cells were produced by passaging primary porcine coronary arterial endothelial cells until passage four. The protein presence of endothelial nitric oxide synthase, cyclic GMP levels [basal and during stimulation (bradykinin and A23187)], were reduced. The mRNA expression level was measured by microarray assays. Genes related to oxidative stress [superoxide dismutase (MnSOD), glutathione peroxidase 3, glutathione S-transferase M1] were downregulated, extracellular matrix components (type III collagen, thrombospondin 1 and 3, transforming growth factor ?) upregulated and nuclear factor kappa B (NF?B)-signaling pathway [IkappaB, TNF receptor-associated factor 1 and 5 (TRAF1 and 5)] activated in senescent cells. The differential gene expression of MnSOD and TRAF5 was confirmed at the protein level by Western blotting and biochemical assay (MnSOD). The basal and stimulated (by tumor necrosis factor-?)?levels of NF?B were augmented as demonstrated by electrophoretic mobility shift assay. In summary, cultured senescent endothelial cells exhibit reduced nitric oxide production, and decreased antioxidative, proliferative capacities, augmented expression of extracellular matrix components and activation of NF?B. These molecular changes do not exactly mimick those occurring during endothelial regeneration in vivo. Experiment Overall Design: Totally 8 samples were analyzed with 4 replicates each for control (cells at passage one) and test (cells at passage four) samples.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8974.idf.txt
Sample and data relationshipE-GEOD-8974.sdrf.txt
Raw data (1)E-GEOD-8974.raw.1.zip
Processed data (1)E-GEOD-8974.processed.1.zip
Array designA-AFFY-75.adf.txt
R ExpressionSetE-GEOD-8974.eSet.r
Links