E-GEOD-8884 - Transcription profiling of human embryonic stem cells, early stage embryoid bodies, and hES-derived blast cells
Submitted on 27 August 2007, released on 16 June 2008, last updated on 27 March 2012
To understand the differentiation process of embryonic stem cells into hemangioblasts, gene expression profiles of ES, EB and Blast cells (BL) were analyzed. Experiment Overall Design: Total RNA was isolated from purified BC cells, day3.5-EBs and undifferentiated ESCs (from two hESC lines, H1 and H9) using the Qiagen RNAeasy kit We sought to obtain homogeneous populations of cells at each developmental stage in order to increase the purity of each sample.
transcription profiling by array, unknown experiment type
GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes. Shi-Jiang Lu, Jennifer A Hipp, Qiang Feng, Jason D Hipp, Robert Lanza, Anthony Atala. Genome Biol 8(11):R240 (2007)