E-GEOD-8739 - Transcription profiling by array of Arabidopsis mutant for ga1 after treatment with GA4
Released on 16 June 2008, last updated on 30 April 2015
The aim is to identify early gibberellin responsive genes in a gibberellin deficient strain such as ga1-3. Such genes are likely regulated by DELLA proteins which are master gibberellin repressors. DELLA proteins are rapidly degraded after gibberellin treatment, but their direct target genes still need to be elucidated. Experiment Overall Design: A set of 4 biological replicates was generated for each treament. Arabidopsis seedlings were treated with water or 2 uM GA4 and whole shoots collected after 1h. A comparison of water vs. GA4 treated samples should render a list of early gibberellin responsive genes.
transcription profiling by array, unknown experiment type
Global analysis of della direct targets in early gibberellin signaling in Arabidopsis. Rodolfo Zentella, Zhong-Lin Zhang, Mehea Park, Stephen G Thomas, Akira Endo, Kohji Murase, Christine M Fleet, Yusuke Jikumaru, Eiji Nambara, Yuji Kamiya, Tai-Ping Sun. , Europe PMC 17933900