E-GEOD-8733 - Transcription profiling of mouse skin from K6/ODC animals response to folate deficient diets reveals folate deficiency enhances arsenic effects
Submitted on 9 August 2007, released on 16 June 2008, last updated on 27 March 2012
We demonstrate that expression of key markers of keratinocyte differentiation is suppressed by exposure to sodium arsenite. Folate deficiency exacerbates this effect. In addition, cancer-related cell movement genes, and growth and proliferation genes are altered. Several redox-sensitive transcription factors are implicated in mediating these gene expression changes due to arsenic treatment and folate deficiency. Experiment Overall Design: K6/ODC mice were maintained on either a folate deficient or folate sufficient diet and exposed to 0, 1, or 10 ppm sodium arsenite in the drinking water for 30 days. Total RNA was isolated from skin samples and gene expression analyzed using Affymetrix Mouse 430 2.0 GeneChips. Data from 24 samples, with four mice in each of the six treatment groups, were analyzed.
transcription profiling by array, unknown experiment type
Folate deficiency enhances arsenic effects on expression of genes involved in epidermal differentiation in transgenic K6/ODC mouse skin. Gail M Nelson, Gene J Ahlborn, Don A Delker, Kirk T Kitchin, Thomas G O'Brien, Yan Chen, Michael J Kohan, Barbara C Roop, William O Ward, James W Allen. Toxicology 241(3):134-45 (2007)