E-GEOD-8730 - Transcription profiling of human pulp and odontoblasts reponse to TGF-beta1
Released on 16 June 2008, last updated on 2 May 2014
Transforming growth factor beta 1 (TGF-β1) is the most extensively studied growth factor in dentin-pulp complex, with pleiotropic effects on pulp response and healing. Our main objective was to analyze the expression profile of pulp tissue and odontoblasts, and the effects of TGF-β1 on these profiles in cultured human pulp and odontoblasts with a specific interest in the anti- and pro-inflammatory cytokines. Experiment Overall Design: Pulp tissues and odontoblasts were cultured for different time periods, and microarray was performed to both cultured and native samples to detect the effects of TGF-β1. Expression of various interleukins (IL) were confirmed by RT-PCR, and in +/- TGF-β1 treated pulps also by antibody array.
transcription profiling by array, unknown experiment type
Effects of TGF-beta1 on interleukin profile of human dental pulp and odontoblasts. Virve Pääkkönen, Jussi Vuoristo, Tuula Salo, Leo Tjäderhane.