E-GEOD-8712 - Transcription profiling of barley response to Yariv-reagent effect on aleurone GA signaling
Submitted on 7 August 2007, released on 16 June 2008, last updated on 10 June 2011
Microarray analysis was performed to know how many gibberellin (GA)-responsive genes are inhibited by beta-Yariv reagent, a specific binder of plant arabinogalactan-proteins. cRNAs were prepared from mRNAs isolated from aleurone protoplasts that were treated with GA, GA plus beta-Yariv reagent, or mock (DMSO)-treated for 24 hours, and were subjected to microarray analysis. The analysis was performed twice using target cRNAs prepared independently. Experiment Overall Design: 2 control (DMSO), 2 gibberellin and 2 gibberellin plus beta-Yariv reagent-treated biological replicates were analyzed at 24 h.
transcription profiling by array, unknown experiment type