E-GEOD-8712 - Transcription profiling of barley response to Yariv-reagent effect on aleurone GA signaling

Status
Submitted on 7 August 2007, released on 16 June 2008, last updated on 10 June 2011
Organism
Hordeum vulgare
Samples (6)
Array (1)
Protocols (4)
Description
Microarray analysis was performed to know how many gibberellin (GA)-responsive genes are inhibited by beta-Yariv reagent, a specific binder of plant arabinogalactan-proteins. cRNAs were prepared from mRNAs isolated from aleurone protoplasts that were treated with GA, GA plus beta-Yariv reagent, or mock (DMSO)-treated for 24 hours, and were subjected to microarray analysis. The analysis was performed twice using target cRNAs prepared independently. Experiment Overall Design: 2 control (DMSO), 2 gibberellin and 2 gibberellin plus beta-Yariv reagent-treated biological replicates were analyzed at 24 h.
Experiment types
transcription profiling by array, unknown experiment type
Contact
kiyoshi mashiguchi
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8712.idf.txt
Sample and data relationshipE-GEOD-8712.sdrf.txt
Raw data (1)E-GEOD-8712.raw.1.zip
Processed data (1)E-GEOD-8712.processed.1.zip
Array designA-AFFY-31.adf.txt
R ExpressionSetE-GEOD-8712.eSet.r
Links