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E-GEOD-86020 - Gene expression profiles of GPR15 knock out macrophages

Status
Released on 25 August 2016, last updated on 12 September 2016
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
To confirm the changed gene expression profiles in GPR15 knock out (KO) macrophages, we applied a SurePrint G3 Mouse Gene Expression service from Takara Bio Inc (Kusatsu, Shiga, Japan) to analyze gene expression profiles in lipopolysaccharide (LPS)-stimulated GPR15KO macrophages, comparing with wild type (WT) macrophages. Most significantly up-regulated genes in GPR15KO macropahges included Il6, Il17a, Il23, Tnfsf8, Il1b, Ifna2 and Ccnd2. On the contrary, several inflammation-related genes, including Ccl17, Itgax, Nrp1 and Rasgrf2, were down-regulated in WT macrophages, compared to GPR15 KO macrophages. Abdominal macrophages from WT and GPR15 KO mice were stimulated with PBS or LPS (100 ng/ml) for 4 hrs. Total RNA were extracted using a TRIzol-chloroform based method.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-86020.idf.txt
Sample and data relationshipE-GEOD-86020.sdrf.txt
Raw data (1)E-GEOD-86020.raw.1.zip
Processed data (1)E-GEOD-86020.processed.1.zip
Additional data (1)E-GEOD-86020.additional.1.zip
Array designA-GEOD-21810.adf.txt
Links