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E-GEOD-8597 - Transcription profiling by array of human MCF7 breast cancer cells after growth with cyclohexamide followed by treatment with 17beta-estradiol

Status
Released on 16 June 2008, last updated on 30 April 2015
Organism
Homo sapiens
Samples (16)
Array (1)
Protocols (6)
Description
Estrogen receptors (ERs), which mediate the proliferative action of estrogens in breast cancer cells, are ligand-dependent transcription factors that regulate expression of their primary target genes through several mechanisms. In addition to direct binding to cognate DNA sequences, ERs can be recruited to DNA through other transcription factors (tethering), or affect gene transcription through modulation of signaling cascades by non-genomic mechanisms of action. To better characterize the mechanisms of gene regulation by estrogens, we have identified more than 700 putative primary and more than 1500 putative secondary target genes of estradiol in MCF7 cells through microarray analysis performed in the presence or absence of the translation inhibitor cycloheximide. Experiment Overall Design: RNA samples were collected 24 h after treatment of MCF7 cells with vehicle or 17{beta}-estradiol (25 nM). Cells were pre-treated 1 h before E2 stimulation with cycloheximide (CHX, 10 microg/ml). Microarray analysis was performed with four replicates for each condition.
Experiment types
transcription profiling by array, compound based treatment
Contact
Citation
Mechanisms of primary and secondary estrogen target gene regulation in breast cancer cells. Bourdeau V, Deschênes J, Laperrière D, Aid M, White JH, Mader S.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8597.idf.txt
Sample and data relationshipE-GEOD-8597.sdrf.txt
Raw data (1)E-GEOD-8597.raw.1.zip
Processed data (1)E-GEOD-8597.processed.1.zip
Array designA-AFFY-44.adf.txt
Links