E-GEOD-8503 - siRNA of mouse undifferentiated Dicer -/- (27H10) embryonic stem cells

Status
Submitted on 17 July 2007, released on 16 June 2008, last updated on 2 May 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (5)
Description
We have analyzed the transcript expression levels in Dicer knock-out embryonic stem (ES) cells 24 hours after transfection with either control siRNA agains Renilla luciferase or miRNA Mimics (Dharmacon) of mmu-miR-290 cluster members in order to identify primary targets of miR-290 cluster miRNAs. Experiment Overall Design: Cell were analysed 24h after transfections in an undifferentiated state. Triplicates of both transfections were analyzed.
Experiment types
RNAi profiling by array, unknown experiment type
Contact
Citation
MicroRNAs control de novo DNA methylation through regulation of transcriptional repressors in mouse embryonic stem cells. Lasse Sinkkonen, Tabea Hugenschmidt, Philipp Berninger, Dimos Gaidatzis, Fabio Mohn, Caroline G Artus-Revel, Mihaela Zavolan, Petr Svoboda, Witold Filipowicz. Nat Struct Mol Biol 15(3):259-67 (2008)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8503.idf.txt
Sample and data relationshipE-GEOD-8503.sdrf.txt
Raw data (1)E-GEOD-8503.raw.1.zip
Processed data (1)E-GEOD-8503.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-8503.eSet.r
Links