E-GEOD-8472 - Chip-chip from pro-B cells with H3K4me3, H3K4me2 and H3K9ac

Status
Released on 23 August 2007, last updated on 2 May 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (14)
Description
The transcription factor Pax5 represses B-lineage-inappropriate genes and activates B-cell-specific genes in B-lymphocytes. Here we have identified 170 novel Pax5-activated genes. Conditional mutagenesis demonstrated that the Pax5-regulated genes require continuous Pax5 activity for normal expression in pro-B and mature B cells. Expression of half of the Pax5-activated genes is either absent or significantly reduced upon Pax5 loss in plasma cells. Direct Pax5 target genes were identified based on their protein synthesis-independent activation by a Pax5-estrogen receptor fusion protein. Chromatin immunoprecipitation (ChIP) of Pax5 together with chromatin profiling by ChIP-on-chip analysis demonstrated that Pax5 directly activates the chromatin at promoters or putative enhancers of Pax5 target genes. The novel Pax5-activated genes code for key regulatory and structural proteins involved in B cell signaling, adhesion, migration, antigen presentation and germinal center B cell formation, thus revealing a complex regulatory network, which is activated by Pax5 to control B cell development and function. Keywords: Chip-chip, cell type comparison comparison of Pax5-/-Rag2-/- vs Rag2-/- pro-B cells
Experiment type
ChIP-chip by tiling array 
Contacts
Meinrad Busslinger, Shane McManus
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8472.idf.txt
Sample and data relationshipE-GEOD-8472.sdrf.txt
Processed data (1)E-GEOD-8472.processed.1.zip
Array designA-GEOD-5532.adf.txt
Links