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E-GEOD-84693 - Interferon-stimulated gene expression in STAT1 knockout cells

Released on 22 July 2016, last updated on 31 July 2016
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
STAT1 plays a cental role in the induction of interferon-stimulated genes, but interferon alpha can activate a STAT1-independent pathway that leads to gene expression. We performed microarray analysis to examine whether like interferon alpha, interferon lambda, a newly discovered interferon, can induce the expression of interferon-stimulated genes in the absence of STAT1. Control and STAT1 knockout Huh-7.5 hepatoma cells were left untreated or treated with 1000 U/ml of human interferon alpha 2a or interferon lambda 1 (PBL) for 24 h, and total RNA was extracted. Sense-strand DNA was generated from 200 ng of total RNA, fragmented, and labeled using a GeneChip WT Plus Reagent Kit (Affymetrix). Six samples were obtained and each sample was anlyzed using one GeneChip.
Experiment type
transcription profiling by array 
Kiyonao Sada, Shota Yamauchi
Investigation descriptionE-GEOD-84693.idf.txt
Sample and data relationshipE-GEOD-84693.sdrf.txt
Raw data (1)
Processed data (1)
Array designA-GEOD-16686.adf.txt