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E-GEOD-84693 - Interferon-stimulated gene expression in STAT1 knockout cells

Status
Released on 22 July 2016, last updated on 31 July 2016
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
STAT1 plays a cental role in the induction of interferon-stimulated genes, but interferon alpha can activate a STAT1-independent pathway that leads to gene expression. We performed microarray analysis to examine whether like interferon alpha, interferon lambda, a newly discovered interferon, can induce the expression of interferon-stimulated genes in the absence of STAT1. Control and STAT1 knockout Huh-7.5 hepatoma cells were left untreated or treated with 1000 U/ml of human interferon alpha 2a or interferon lambda 1 (PBL) for 24 h, and total RNA was extracted. Sense-strand DNA was generated from 200 ng of total RNA, fragmented, and labeled using a GeneChip WT Plus Reagent Kit (Affymetrix). Six samples were obtained and each sample was anlyzed using one GeneChip.
Experiment type
transcription profiling by array 
Contacts
Kiyonao Sada, Shota Yamauchi
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-84693.idf.txt
Sample and data relationshipE-GEOD-84693.sdrf.txt
Raw data (1)E-GEOD-84693.raw.1.zip
Processed data (1)E-GEOD-84693.processed.1.zip
Array designA-GEOD-16686.adf.txt
Links