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E-GEOD-8248 - Transcription profiling by array of Arabidopsis after hypoxia treatment

Released on 16 June 2008, last updated on 5 January 2012
Arabidopsis thaliana
Samples (2)
Array (1)
Protocols (7)
The goal of this experiment was to investigate the early transcript changes (6h) induced by hypoxia treatment in mesophyll protoplasts. A single pair (control & hypoxia) of GeneChips® was used to confirm that hypoxia treatment altered the expression of an overlapping set of genes controlled by KIN10 (At3g01090) in Arabidopsis mesophyll protoplasts. Experiment Overall Design: For the hypoxic treatment protoplasts were submerged in mannitol buffer and the hypoxia condition was confirmed by the activation of the well-established early marker genes ADH (alcohol dehydrogenase, At1g77120) and PDC1 (pyruvate decarboxylase-1, At4g33070) based on RT-PCR and GeneChip® analyses. Results show a clear overlap in genes induced under hypoxic conditions and those induced by KIN10 expression in protoplasts. The overlap of the gene expression profiles was confirmed by triplicated real-time quantitative RT-PCR analyses of selected marker genes.
Experiment types
transcription profiling by array, unknown experiment type
A central integrator of transcription networks in plant stress and energy signalling. Elena Baena-González, Filip Rolland, Johan M Thevelein, Jen Sheen. , PMID:17671505
Investigation descriptionE-GEOD-8248.idf.txt
Sample and data relationshipE-GEOD-8248.sdrf.txt
Raw data (1)
Processed data (1)
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-8248.eSet.r