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E-GEOD-8248 - Transcription profiling by array of Arabidopsis after hypoxia treatment

Status
Released on 16 June 2008, last updated on 5 January 2012
Organism
Arabidopsis thaliana
Samples (2)
Array (1)
Protocols (7)
Description
The goal of this experiment was to investigate the early transcript changes (6h) induced by hypoxia treatment in mesophyll protoplasts. A single pair (control & hypoxia) of GeneChips® was used to confirm that hypoxia treatment altered the expression of an overlapping set of genes controlled by KIN10 (At3g01090) in Arabidopsis mesophyll protoplasts. Experiment Overall Design: For the hypoxic treatment protoplasts were submerged in mannitol buffer and the hypoxia condition was confirmed by the activation of the well-established early marker genes ADH (alcohol dehydrogenase, At1g77120) and PDC1 (pyruvate decarboxylase-1, At4g33070) based on RT-PCR and GeneChip® analyses. Results show a clear overlap in genes induced under hypoxic conditions and those induced by KIN10 expression in protoplasts. The overlap of the gene expression profiles was confirmed by triplicated real-time quantitative RT-PCR analyses of selected marker genes.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
A central integrator of transcription networks in plant stress and energy signalling. Elena Baena-González, Filip Rolland, Johan M Thevelein, Jen Sheen. , PMID:17671505
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8248.idf.txt
Sample and data relationshipE-GEOD-8248.sdrf.txt
Raw data (1)E-GEOD-8248.raw.1.zip
Processed data (1)E-GEOD-8248.processed.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-8248.eSet.r
Links