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E-GEOD-8229 - Transcription profiling of human U937 leukemia cells in apoptosis induced by ansimycin

Status
Submitted on 21 June 2007, released on 16 June 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (5)
Array (1)
Protocols (4)
Description
Anisomycin is known as a potent apoptosis inducer by activating JNK/SAPK and inhibiting protein synthesis during translation. However, only few details are known on the mechanism of apoptosis induced by this compound. Genes in apoptosis induced by anisomycin in human leukemia U937 cells were investigated by using an Affymetrix GeneChip system. DNA fragmentation and phosphatidylserine externalization assays clearly demonstrated that anisomycin induced apoptosis in a time- and concentration- dependent manner. Of 22,283 probe sets analyzed, this compound down-regulated 524 probe sets and up-regulated 523 by a factor 1.5 or greater. Experiment Overall Design: U937 cells, a human lymphoma cell line, were incubated with anisomycin (1 μM) for 0, 1, 2, 3, and 6 h at 37°C. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with Human Expression Array U133A which was spotted with 22,283 probe sets. Sample preparation for array hybridization was carried out as described in the manufacture’s instructions.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Molecular mechanism of apoptosis and gene expressions in human lymphoma U937 cells treated with anisomycin. Kondo Hori, Takasaki Tabuchi, Kanamori Zhao, Kimura Yasuda. Chem Biol Interact 172(2):125-140 (2008)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8229.idf.txt
Sample and data relationshipE-GEOD-8229.sdrf.txt
Raw data (1)E-GEOD-8229.raw.1.zip
Processed data (1)E-GEOD-8229.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-8229.eSet.r
Links