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E-GEOD-81220 - The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development
Released on 19 August 2016, last updated on 12 September 2016
Wingless (Wg)/Wnt signaling is conserved in all metazoan animals and plays critical roles in development. The Wg/Wnt morphogen reception is essential for signal activation, whose activity is mediated through the receptor complex and a scaffold protein Dishevelled (Dsh). We report here that the exon junction complex (EJC) activity is indispensable for Wg signaling by maintaining an appropriate level of Dsh protein for Wg ligand reception in Drosophila. Transcriptome analyses in Drosophila wing imaginal discs indicate that the EJC controls the splicing of the cell polarity gene disc large 1 (dlg1), whose coding protein directly interacts with Dsh. Genetic and biochemical experiments demonstrate that Dlg1 protein acts independently from its role in cell polarity to protect Dsh protein from lysosomal degradation. More importantly, human orthologous Dlg protein is sufficient to promote Dvl protein stabilization and Wnt signaling activity, thus revealing a conserved regulatory mechanism of Wg/Wnt signaling by Dlg and EJC. whole transcriptome RNA-seq to examine mRNAs extracted from wildtype (i.e. overexpressing lacZ) and pre-EJC-defective (i.e. overexpressing tsu RNAi) wing discs, respectively.
RNA-seq of coding RNA
Ruifeng Li <firstname.lastname@example.org>, Alan J Zhu, Cheng Li, Min Liu, Yajuan Li
The exon junction complex regulates the splicing of cell polarity gene dlg1 to control Wingless signaling in development. Liu M, Li Y, Liu A, Li R, Su Y, Du J, Li C, Zhu AJ. , PMID:27536874