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E-GEOD-8087 - RNAi profiling of human MDA-MB-231 cells knocked down for RhoGDIbeta

Status
Submitted on 12 June 2007, released on 15 June 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (5)
Description
RhoGDIbeta (ARHGDIB) is often expressed in tumor cells. It negatively regulates Rho-GTPases, but may have other functions as well. To analyze its effect on gene expression, RhoGDIbeta was suppressed by RNA interference in MDA-MB-231 breast cancer cells and changes in gene expression monitored by cDNA microarrays. Experiment Overall Design: MDA-MB-231 cells were either transfected with a RhoGDIbeta-specific siRNA or a control siRNA (siLuc), grown for three days, harvested and lysed for total RNA isolation. RNA was subjected to cDNA microarray analysis by using Affymetrix chip HG-U133A. A total of three independent transfection experiments were performed. The samples obtained from experiments 1, 2 and 3 were designated as siLuc_rep1, siLuc_rep2, siLuc_rep3 (siLuc transfected) and siARH_rep1, siARH_rep2, siARH_rep3 (siRhoGDIbeta transfected), respectively.
Experiment types
RNAi profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-8087.idf.txt
Sample and data relationshipE-GEOD-8087.sdrf.txt
Raw data (1)E-GEOD-8087.raw.1.zip
Processed data (1)E-GEOD-8087.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-8087.eSet.r
Links