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E-GEOD-79646 - Expression data from Drosophila mutants´ (misexpression and overexpression) of cabut/dTIEG gene

Status
Released on 29 March 2016, last updated on 3 April 2016
Organism
Drosophila melanogaster
Samples (9)
Array (1)
Protocols (5)
Description
cabut (cbt), is the Drosophila ortholog of the human TGF-β Inducible Early Gene 1 (dTIEG). It encodes a transcription factor involved in Drosophila dorsal closure (DC), and it is expressed in embryonic epithelial sheets and yolk cell during this process upon activation of the Jun N-terminal kinase (JNK) signaling pathway. Additional studies suggest that cbt may have a role in multiple developmental processes. We used microarrays to detail the target genes of Cbt during dorsal closure process cbtEP(2)2237E1 homozigote Drosophila mutant embryos were selected at 10-13 stage (during dorsal closure process) from non-GFP embryos from the line cbtEP(2)2237E1/Cy0, twi-eGFP line by embryo sorter assays. In parallel, samples of white embryos at same stages (as control) were sorting also by embryo sorter. Also using UAS-GAL4 system, Cbt was overexpressed in the embrionic epidermis during 10-13 stage by epidermal driver (69B-Gal4) and UAS-Cbt line.
Experiment type
transcription profiling by array 
Contacts
YAIZA BELACORTU <bepasyai@uv.es>, Nuria Paricio, Yaiza Belacortu
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-79646.idf.txt
Sample and data relationshipE-GEOD-79646.sdrf.txt
Raw data (1)E-GEOD-79646.raw.1.zip
Processed data (1)E-GEOD-79646.processed.1.zip
Array designA-AFFY-35.adf.txt
Links