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E-GEOD-79450 - Expression data from infant ALL with MLL-AF4 rearrangement

Status
Released on 22 March 2016, last updated on 26 March 2016
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (7)
Description
MLL-AF4+ blasts from infant B-ALL, CB-derived CD34+CD38-CD19-CD33- HSC, CB-derived CD34+CD19+CD33- B-cell HPCs and CB-derived CD34+CD33+CD19- myeloid HPCs. We used microarray to estudy gene expression profile comparing ALL vs HSC, HPC and myeloid HPSC. We used highly FACS-purified (purity>98%) MLL-AF4+ blasts from infant B-ALL, CB-derived CD34+CD38-CD19-CD33- HSC, CB-derived CD34+CD19+CD33- B-cell HPCs and CB-derived CD34+CD33+CD19- myeloid HPCs. For each independent sample technical duplicates were always performed. Total RNA was extracted using TRIol reagent, and quantified on a Nanodrop spectrophotometer and Bioanalyzer. High-quality RNA was reverse transcribed and the obtained cDNA was used as a template to synthesize biotinylanted cDNA, then was fragmented and hybridized as duplicates/triplicates to HG-U133 plus2.0 GeneChips (Affymetrix) according to manufacturer's guideline.
Experiment type
transcription profiling by array 
Contacts
antonio agraz <antonio.agraz@unican.es>, Gianni Cazzaniga, Michela Bardini
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-79450.idf.txt
Sample and data relationshipE-GEOD-79450.sdrf.txt
Raw data (1)E-GEOD-79450.raw.1.zip
Processed data (1)E-GEOD-79450.processed.1.zip
Array designA-AFFY-44.adf.txt
Links