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E-GEOD-79063 - Plasma-specific microRNA responses in rats treated with acute toxicity doses of aristolochic acid I

Status
Released on 10 March 2016, last updated on 20 March 2016
Organism
Rattus norvegicus
Samples (20)
Array (1)
Protocols (6)
Description
In the present study, goal was to scan the potential biomarker for acute kidney injury induced by aristolochic acid I (AAI).We utilized the microarry analysis to investigate the microRNA (miRNA) expression profile in kidneys from rat treated by 40mg/kg AA I for 2-6 days. miRNAs with significantly different expression of global miRNA expression profile were validated by qRT-PCR. For miRNAs still significantly disregulation, we further examined the expression in plasma of rats treated with AAI dosed at 10, 20 and 40mg/kg AAI for 2-6 days by qRT-PCR. miRNAs with significantly dysregulation in plasma, their expression in brain, liver and heart was examined for kicking out the non-specific disregulation in AAI induced acute kidney injury, so that the significant dysregulation miRNAs with specificity in kidney and plasma was found as potential biomarkers for AAI induced acute kidney injury. Five control and 15 kidneys treated with 40mg/kg AAI on day 2, 4 and 6 was examined by microarray.
Experiment type
transcription profiling by array 
Contacts
Ze'an Zhang <zza@shutcm.edu.cn>, Jiaying Shen, Zean Zhang, Zhongping Deng
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-79063.idf.txt
Sample and data relationshipE-GEOD-79063.sdrf.txt
Raw data (1)E-GEOD-79063.raw.1.zip
Processed data (1)E-GEOD-79063.processed.1.zip
Array designA-GEOD-18115.adf.txt
Links