E-GEOD-7899 - Transcriptional profiling of inductive prostatic mesenchyme
Submitted on 24 May 2007, released on 20 December 2007, last updated on 27 March 2012
Background: The mesenchymal compartment plays a key role in organogenesis and cells within the mesenchyme/stroma are a source of potent molecules that control epithelia during development and tumourigenesis. We have used Serial Analysis of Gene Expression (SAGE) to profile a key subset of prostatic mesenchyme that regulates prostate development and is enriched for growth-regulatory molecules. Results: SAGE libraries were made of prostatic inductive mesenchyme (VMP) and the complete prostatic rudiment (including inductive mesenchyme, epithelium and smooth muscle; VSU). By comparing these two SAGE libraries we generated a list of 219 transcripts that were enriched or specific to inductive mesenchyme and which may act as mesenchymal regulators of organogenesis and tumourigenesis. Conclusions: The use of a precisely defined subset of cells, and a back-comparison approach, allowed us to identify rare mRNAs that might be overlooked using other approaches. Keywords: SAGE, gene profiling Two SAGE libraries were prepared and compared to each other. The VMP forms a subset of the VSU.
transcription profiling by SAGE
Axel Thomson <firstname.lastname@example.org>, Axel A Thomson, Brigid Orr
Transcriptional profiling of inductive mesenchyme to identify molecules involved in prostate development and disease. Vanpoucke G, Orr B, Grace OC, Chan R, Ashley GR, Williams K, Franco OE, Hayward SW, Thomson AA.