E-GEOD-7896 - Transcription profiling of human male embryonic stem cell line line grown in standard conditions, with feeder cells and treated with the signalling sphingolipid sphingosine-1-phosphate reveals S1P mediates key targets associated with survival, proliferation and pluripotency

Status
Submitted on 24 May 2007, released on 30 April 2009, last updated on 27 March 2012
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (5)
Description
Human embryonic stem cells (hESCs) replicate by the process of self-renewal, whilst maintaining their pluripotency. Understanding the pathways involved in the regulation of this self-renewal process will assist in developing fully-defined conditions for the proliferation of hESCS required for therapeutic applications. We previously demonstrated a role for Sphingosine-1-phosphate (S1P) in the survival and proliferation of hESCs. The present study investigates further key signalling pathways and the downstream targets of S1P. Microarrays were used to examine changes in gene expression invoked by treatment of human embryonic stem cells grown upon different matrices Experiment Overall Design: Human embryonic stem cells (Sheff 4) were grown upon MEFs. One group were treated with S1P. Each group consisted of three replicates which were hybridised to Human U133 plus 2 Affymetrix GeneChips
Experiment types
transcription profiling by array, compound treatment, growth condition
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7896.idf.txt
Sample and data relationshipE-GEOD-7896.sdrf.txt
Raw data (1)E-GEOD-7896.raw.1.zip
Processed data (1)E-GEOD-7896.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-7896.eSet.r
Links