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E-GEOD-7891 - Transcription profiling of rat inner medullary collecting duct

Status
Submitted on 24 May 2007, released on 15 June 2008, last updated on 2 May 2014
Organism
Rattus norvegicus
Samples (6)
Array (1)
Protocols (6)
Description
Identification of gene expressed in the enriched inner medullary collecting duct cells in rat. Experiment Overall Design: Rat inner medullary collecting duct (IMCD) cells were isolated from 7 male Sprage-Dawley rats by collagenase and hyaluronidase digestion and follow by low speed centrifugation. The non-IMCD cells were collected by centrifugation of supernatant of enriched IMCD samples. Experiment Overall Design: Total RNA about 3 ug were used per microarray (Rat 230 2.0 Genechip array). Experiment Overall Design: The experiments were repeat 3 times (3 pairs of IMCD VS non-IMCD)
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Transcriptional profiling of native inner medullary collecting duct cells from rat kidney. Panapat Uawithya, Trairak Pisitkun, Brian E Ruttenberg, Mark A Knepper. Physiol Genomics 32(2):229-53 (2008)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7891.idf.txt
Sample and data relationshipE-GEOD-7891.sdrf.txt
Raw data (1)E-GEOD-7891.raw.1.zip
Processed data (1)E-GEOD-7891.processed.1.zip
Array designA-AFFY-43.adf.txt
R ExpressionSetE-GEOD-7891.eSet.r
Links