E-GEOD-7888 - Transcription profiling of human mesenchymal stem cells (six batches)
Submitted on 24 May 2007, released on 18 June 2008, last updated on 10 June 2011
Human mesenchymal stem cells (hMSCs), which are multipotent cells to differentiate into several cell types, are expected to be a useful tool for cellular therapy. In some clinical settings, hMSCs have immuno-suppressive effects for GVHD (Graft-versus-host disease) and are expanded in vitro before application. To find biomarkers that indicate the culture stage of hMSCs, we performed microarray analysis for hMSCs derived from bone marrow, using Affymetrix GeneChip Human Genome U133 Plus 2.0 (54,613 probe sets). Experiment Overall Design: We profiled the gene expression in 6 different batches of hMSCs. The cells were cultured and subjected to total RNA extraction. We performed microarray analysis using 6 batches provided by 6 different individuals in early stage (passage #4 or 5), middle stage (#7 or 9) or senescing stage (#22, 24, or 28).
transcription profiling by array, unknown experiment type