E-GEOD-7888 - Transcription profiling of human mesenchymal stem cells (six batches)

Status
Submitted on 24 May 2007, released on 18 June 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (23)
Array (1)
Protocols (4)
Description
Human mesenchymal stem cells (hMSCs), which are multipotent cells to differentiate into several cell types, are expected to be a useful tool for cellular therapy. In some clinical settings, hMSCs have immuno-suppressive effects for GVHD (Graft-versus-host disease) and are expanded in vitro before application. To find biomarkers that indicate the culture stage of hMSCs, we performed microarray analysis for hMSCs derived from bone marrow, using Affymetrix GeneChip Human Genome U133 Plus 2.0 (54,613 probe sets). Experiment Overall Design: We profiled the gene expression in 6 different batches of hMSCs. The cells were cultured and subjected to total RNA extraction. We performed microarray analysis using 6 batches provided by 6 different individuals in early stage (passage #4 or 5), middle stage (#7 or 9) or senescing stage (#22, 24, or 28).
Experiment types
transcription profiling by array, unknown experiment type
Contact
Shihori Tanabe
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7888.idf.txt
Sample and data relationshipE-GEOD-7888.sdrf.txt
Processed data (1)E-GEOD-7888.processed.1.zip
Array designA-AFFY-44.adf.txt
Links