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E-GEOD-7884 - INDUCTION OF PANCREATIC ISLET CELL DIFFERENTIATION BY THE NEUROGENIN-NEUROD CASCADE

Status
Released on 29 August 2007, last updated on 2 May 2014
Organism
Mus musculus
Samples (16)
Array (1)
Protocols (20)
Description
Neurog3 and NeuroD1 share the ability to induce endocrine differentiation when expressed ectopically in vivo (Apelqvist et al., 1999; Schwitzgebel et al., 2000; Grapin-Botton et al., 2001) and in vitro (Heremans et al., 2002; Gasa et al., 2004), suggesting that they target a common set of genes. To test this hypothesis, we directly compared in the same cells the effects of Neurog3 and NeuroD1 on global gene expression patterns. Normalized M and A values [limmaGUI output] for the neurogenin3 (Ngn3) and neuroD1 (Nd1) experiments (individual or combined) have been linked below as Supplementary files. Note that M=log2(test/ref), eg, log2(Nd1/Bgal); A=[log2(test*ref)]/2, eg, [log2(Nd1*Bgal)]/2. Additional measures of reliability/probability are also included within the supplementary files. Mouse pancreatic ductal cells (mPAC cells) were infected with equal titers of recombinant adenoviruses encoding human Neurogenin3 (AdCMV-NEUROG3) or mouse NeuroD1 (AdCMV-NeuroD1), and 48 hrs later their transcriptomes were compared with that of control cells infected with an adenovirus encoding betagalactosidase (beta-gal; AdCMV-Bgal) using cDNA microarrays. Differences in the activation patterns of the two transcription factors were then determined by comparing the fold-change (FC) values of the pair-wise hybridizations Neurog3/Bgal and NeuroD1/beta-gal.
Experiment type
unknown experiment type 
Contacts
Michael Scott German <mgerman@diabetes.ucsf.edu>, Caroline MREJEN, Chin-Hsing LING, Francis C LYNN, Katherine YANG, Lidia SANCHEZ, Michael S GERMAN, Peter SKEWES-COX, Ramon GOMIS, Rosa GASA
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7884.idf.txt
Sample and data relationshipE-GEOD-7884.sdrf.txt
Processed data (1)E-GEOD-7884.processed.1.zip
Array designA-GEOD-1884.adf.txt
Links