E-GEOD-7874 - Transcription profiling of human CD34+ treated with EPO at day 7 and 14 to investigate the effect of EPO on erythroid maturation
Submitted on 22 May 2007, released on 15 June 2008, last updated on 10 June 2011
To study transcriptional profiles in human CD34+ cell in EPO condition on day 7 and day 14. Experiment Overall Design: After obtaining informed consent, human CD34+ cells were isolated in high purity from the peripheral blood of normal human volunteers. The cells were cultured at a concentration of 104 -105 cells/mL in medium supplemented with 4 U/mL EPO (Amgen, Thousand Oaks, CA) as described previously.1 Cells were enumerated using an electronic cell counter (Coulter, Hialeah, FL). Adult and fetal hemoglobin was analysed by HPLC. RNA was extracted using QIAshredder and Rneasy Minikit.
transcription profiling by array, unknown experiment type