E-GEOD-787 - Corticosteroid responsive genes

Status
Submitted on 30 October 2003, released on 30 October 2003, last updated on 27 March 2012
Organism
Rattus norvegicus
Samples (3)
Protocols (1)
Description
Three groups of Wistar rats with different manipulated levels of corticosterone were used to identify corticosteroid-responsive genes in hippocampus: 1. rats were adrenalectomised (ADX) 2. In addition to ADX these rats were implanted with a subcutaneous pellet containing 20 mg corticosterone and 80% cholesterol (ADX + low CORT) 3. In addition to ADX and implantation of a corticosterone pellet, these rats received a subcutaneous injection with corticosterone (1 mg/kg bodyweight) three hours prior to decapitation ((ADX + low CORT) + acute high CORT). All 3 groups of rats were decapitated three days after ADX and their hippocampus was isolated. Hippocampal RNA from 6 rats per group was pooled and used as input material for the SAGE libraries. Keywords: parallel sample
Experiment type
transcription profiling by SAGE 
Contacts
Nicole Datson <datson_n@lacdr.leidenuniv.nl>, E. Ronald de Kloet, Erno Vreugdenhil, Jeannette van der Perk
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-787.idf.txt
Sample and data relationshipE-GEOD-787.sdrf.txt
Processed data (1)E-GEOD-787.processed.1.zip
Links