E-GEOD-787 - Corticosteroid responsive genes
Submitted on 30 October 2003, released on 30 October 2003, last updated on 27 March 2012
Three groups of Wistar rats with different manipulated levels of corticosterone were used to identify corticosteroid-responsive genes in hippocampus: 1. rats were adrenalectomised (ADX) 2. In addition to ADX these rats were implanted with a subcutaneous pellet containing 20 mg corticosterone and 80% cholesterol (ADX + low CORT) 3. In addition to ADX and implantation of a corticosterone pellet, these rats received a subcutaneous injection with corticosterone (1 mg/kg bodyweight) three hours prior to decapitation ((ADX + low CORT) + acute high CORT). All 3 groups of rats were decapitated three days after ADX and their hippocampus was isolated. Hippocampal RNA from 6 rats per group was pooled and used as input material for the SAGE libraries. Keywords: parallel sample
transcription profiling by SAGE
Nicole Datson <email@example.com>, E. Ronald de Kloet, Erno Vreugdenhil, Jeannette van der Perk
Identification of corticosteroid-responsive genes in rat hippocampus using serial analysis of gene expression. Datson NA, van der Perk J, de Kloet ER, Vreugdenhil E.