E-GEOD-7860 - Non-coding antisense transcription detected by conventional and single-stranded cDNA microarray
Released on 1 September 2007, last updated on 2 May 2014
Recent bioinformatic studies revealed that many mammalian protein-coding genes also transcribe their complementary strands. This phenomenon raises questions regarding the validity of data obtained from double-stranded cDNA microarrays since hybridization to both strands may occur. To analyze experimentally the incidence of antisense transcription in human cells and to estimate their influence on protein coding expression patterns obtained by double-stranded microarrays, we profiled transcription with sense and antisense independently by using strand-specific cDNA microarrays. We modeled an experimental design typically used for tumor classification studies. We performed hybridizations using direct-labeled randomly primed total RNA extracted from eight breast-cancer cell lines and one derived from normal breast epithelium against Universal Human Reference RNA as a common reference following standard protocols.
unknown experiment type
Ake Borg, Anders Kvist, Carlos Rovira, Johan Staaf, Johan Vallon-Christersson, Patrik Medstrand
Non-coding antisense transcription detected by conventional and single-stranded cDNA microarray. Vallon-Christersson J, Staaf J, Kvist A, Medstrand P, Borg A, Rovira C. , Europe PMC 17727707