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E-GEOD-7852 - Transcription profiling of mouse fat derived flow sorted Treg cells

Status
Submitted on 18 May 2007, released on 7 August 2009, last updated on 2 May 2014
Organism
Mus musculus
Samples (18)
Array (1)
Protocols (4)
Description
Comparisons of global gene-expression profiles revealed a greater distinction between CD4+ Treg cells and CD4+ conventional (Tconv) T cells residing in abdominal (epidydimal) fat versus in more standard locations such as the spleen, thymus and LN. Experiment Overall Design: All gene expression profiles were obtained from highly purified T cell populations sorted by flow cytometry. To reduce variability, cells from multiple mice were pooled for sorting, and three replicates were generated for all groups. RNA from 3-5 x 104 cells was amplified, labeled, and hybridized to Affymetrix M430v2 microarrays. Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7852.idf.txt
Sample and data relationshipE-GEOD-7852.sdrf.txt
Raw data (1)E-GEOD-7852.raw.1.zip
Processed data (1)E-GEOD-7852.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-7852.eSet.r
Links