E-GEOD-7844 - Differential DNA methylation profiles in estrogen-pre-exposed breast epithelial cells

Status
Submitted on 17 May 2007, released on 30 November 2007, last updated on 2 May 2014
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (8)
Description
This study tried to determine whether exposure of breast stem/progenitor cells to estrogen disrupts the epigenome of progeny epithelial cells. DNA methylation profiles were compared between control and pre-exposed epithelial cells using a genome-wide detection method called MeDIP-chip. Keywords: MeDIP-chip Breast stem/progenitor cells were continuously exposed to 17beta-estradiol (E2, 70 nM) or DMSO (vehicle control) for two weeks and then placed on 2-dimensional collagen substratum for 2-3 weeks for epithelial cell differentiation. DNA from control and pre-exposed cells were extracted and MeDIP ssays were performed using antibodies against 5-methylcytosine. The immunoprecipitated and input DNA were used to probe the Agilent human CpG island microarray. Dye-swap experiments were also performed.
Experiment type
methylation profiling by array 
Contacts
Citation
Epithelial progeny of estrogen-exposed breast progenitor cells display a cancer-like methylome. Cheng AS, Culhane AC, Chan MW, Venkataramu CR, Ehrich M, Nasir A, Rodriguez BA, Liu J, Yan PS, Quackenbush J, Nephew KP, Yeatman TJ, Huang TH.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7844.idf.txt
Sample and data relationshipE-GEOD-7844.sdrf.txt
Raw data (1)E-GEOD-7844.raw.1.zip
Processed data (1)E-GEOD-7844.processed.1.zip
Array designA-GEOD-5148.adf.txt
R ExpressionSetE-GEOD-7844.eSet.r
Links