E-GEOD-7836 - Transcription profiling of rat REF52 cells expressing SV40 T antigens
Submitted on 17 May 2007, released on 12 September 2009, last updated on 2 May 2014
SV40 transforms cells through the action of two oncoproteins, large T antigen and small t antigen. Small t antigen targets phosphatase PP2A, while large T antigen stimulates cell proliferation and survival by action on multiple proteins, including the tumor suppressors Rb and p53. Large T antigen also binds components of the transcription initiation complex and several transcription factors. We examine global gene expression in SV40-transformed REF52 cells. SV40 transformation alters the expression of approximately 480 cellular genes. Much of this regulation is consistent with T antigen action on the Rb-E2F pathway. Experiment Overall Design: We used the Affymetrix RGU34A microarrays to probe the global mRNA expression from two wild-type REF52neo and three REF52neoT stable cell lines. Cells were grown to two days post-confluence and harvested.
transcription profiling by array, unknown experiment type
Cell-type specific regulation of gene expression by simian virus 40 T antigens. Paul G Cantalupo, Maria Teresa Sáenz-Robles, Abhilasha V Rathi, Rebecca W Beerman, William H Patterson, Robert H Whitehead, James M Pipas.