E-GEOD-7785 - PANC-1 cell differentiation

Status
Submitted on 12 May 2007, released on 15 May 2007, last updated on 2 May 2014
Organism
Homo sapiens
Samples (40)
Arrays (3)
Protocols (35)
Description
Multiple commercial microarrays for measuring genome-wide gene expression levels are currently available, including oligonucleotide and cDNA, single- and two-channel formats. This study reports on the results of gene expression measurements generated from identical RNA preparations that were obtained using three commercially available microarray platforms. RNA was collected from PANC-1 cells grown in serum-rich medium and at 24 h following the removal of serum. Three biological replicates were prepared for each condition, and three experimental replicates were produced for the first biological replicate. RNA was labeled and hybridized to microarrays from three major suppliers according to manufacturers’ protocols, and gene expression measurements were obtained using each platform’s standard software. For each platform, gene targets from a subset of 2009 common genes were compared. Correlations in gene expression levels and comparisons for significant gene expression changes in this subset were calculated, and showed considerable divergence across the different platforms, suggesting the need for establishing industrial manufacturing standards, and further independent and thorough validation of the technology. Keywords: Differentiation PANC-1 cells were grown in serum-containing media followed by exposure to serum-free media for 24 hours. In both groups, RNA extracted from the first biological replicate was derived from 3 culture dishes producing enough sample for three technical replicates, whereas RNA from the second and third biological replicates were derived from one culture dish each.
Experiment type
transcription profiling by array 
Contacts
Citation
Evaluation of gene expression measurements from commercial microarray platforms. Tan PK, Downey TJ, Spitznagel EL Jr, Xu P, Fu D, Dimitrov DS, Lempicki RA, Raaka BM, Cam MC.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7785.idf.txt
Sample and data relationshipE-GEOD-7785.sdrf.txt
Raw data (1)E-GEOD-7785.raw.1.zip
Processed data (1)E-GEOD-7785.processed.1.zip
Array designsA-GEOD-8300.adf.txt, A-MEXP-132.adf.txt, A-MEXP-162.adf.txt
Links