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E-GEOD-7767 - Transcription profiling of mouse C57BL6J and DBA2J strains when Chlamydia infected

Status
Submitted on 9 May 2007, released on 15 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (6)
Description
C57BL/6J mice were 105-fold more resistant to Chlamydia psittaci infection than DBA/2J mice by LD100 determinations. Linkage analysis using BXD recombinant inbred strains revealed a single effector locus at a 1.5 Mbp region on chromosome 11 encoding a cluster of three p47GTPases (Irgb10, Igtp, and Iigp2). Western blots of infected tissue showed that Irgb10 was elevated in resistant mice and one of the two possible Iigp2 protein isoforms was preferentially expressed in susceptible mice. The BXD39 strain, susceptible at Irgb10 and resistant at Iigp2, had an intermediate phenotype, implicating the non-redundant role of these p47GTPases. C57BL/6J and DBA/2J exhibited a difference in IFNg dependent chlamydial control, which was reversible by Iigp2 siRNA knockdown. Microarrays of infected peritoneal lavage revealed >10 fold up regulation of neutrophil recruiting chemokines in susceptible mice and >100 fold increase in macrophage differentiation genes in resistant mice, indicating that susceptibility pattern involves stimulation of different inflammatory cell recruiting pathways. Massive neutrophil recruitment was seen in susceptible mice by histology and flow cytometry, and neutrophil chemokine receptor (CXCR2) knockout mice on a susceptible background survived lethal challenge confirming that neutrophil recruitment was required for susceptibility. Congenic Igtp knockout mice also susceptible at Irgb10 and Iigp2 on a resistant background recruited neutrophils and succumbed to infection. We conclude that Irgb10 and Iigp2 act together to confer differential susceptibility against murine chlamydial infection. Results indicate that these p47GTPases have cell autonomous effects, which results in vastly different inflammatory stimulation leading to either recovery or death. Experiment Overall Design: C57BL/6J and DBA2J mice (4 mice each) were infected I.p. with 10E4 IFU of Chlamydia psittaci then peritoneal lavage was collected on day 3 post infection. Cells were centrifuged then treated with Trizol for total RNA extraction.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
The p47 GTPases Iigp2 and Irgb10 regulate innate immunity and inflammation to murine Chlamydia psittaci infection. Isao Miyairi, Venkat R R Arva Tatireddigari, Olaimatu S Mahdi, Lorne A Rose, Robert J Belland, Lu Lu, Robert W Williams, Gerald I Byrne. J Immunol 179(3):1814-24 (2007)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7767.idf.txt
Sample and data relationshipE-GEOD-7767.sdrf.txt
Raw data (1)E-GEOD-7767.raw.1.zip
Processed data (1)E-GEOD-7767.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-7767.eSet.r
Links