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E-GEOD-7700 - shRNA profiling of human normal breast and breast cancer cell lines to identify genes regulated by YAP

Status
Submitted on 3 May 2007, released on 18 January 2009, last updated on 10 June 2011
Organism
Homo sapiens
Samples (10)
Array (1)
Protocols (5)
Description
Frequent loss of heterozygosity (LOH) at 11q22-23 in breast cancer strongly suggests that this region contains a tumor suppressor gene, yet to be identified. We and others have shown Yes-associated protein (YAP), which is located at 11q22.2, transactivates the p53 family member p73 upon DNA damage, suggesting a tumor suppressive function for YAP. Our analysis of breast tumor tissues by immunohistochemistry (IHC) showed loss of YAP protein expression in great portion of breast cancers. We used microarray to look at the targte genes regulated by YAP in normal breast luminal cell and breast cancer cell lines. Experiment Overall Design: We generated stable cell lines by introducing control vector(pRS-IRES-GFP/pmig) or YAP shRNA(pRS-IRES-GFP-YAP/pmig-YAP) in a normal breast luminal cell line 1089 luminal and breast cancer cell lines MDA MB231. RAN was extracted and hybridized on Affymetrix microarrays.We looked for new target genes regulated by YAP in normal breast luminal cell and breast cancer cell lines.
Experiment types
RNAi profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7700.idf.txt
Sample and data relationshipE-GEOD-7700.sdrf.txt
Raw data (1)E-GEOD-7700.raw.1.zip
Processed data (1)E-GEOD-7700.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-7700.eSet.r
Links