E-GEOD-7699 - Transcription profiling of mouse livers from animals fed a ketogenic diet (KD), a diet high in fat and low in carbohydrates
Submitted on 3 May 2007, released on 13 December 2007, last updated on 2 May 2014
Analysis of liver gene transcription during feeding of a ketogenic diet. Ketogenic diets may alter physiologic and metabolic profiles in a direction that favors weight loss. C57BL/6J mice were maintained for six weeks on either chow or ketogenic diet. Mice eating KD had lower weights, 90% reduction in insulin levels and increased energy expenditure compared to animals fed chow. Despite consumption of a very high fat diet serum lipids remained normal. Here we show that consumption of KD shifted liver metabolism to drastically increased fatty acid oxidation. Concurrently, expression of genes involved in fatty acid synthesis were markedly suppressed. Reference: A high fat, ketogenic diet induces a unique metabolic state in mice. Kennedy AR, Pissios P, Out H, Xue B, Asakura K, Furukawa N, Marino FE, Liu FF, Kahn BB, Liberman TA, Maratos-Flier E. in press, 2007, Am J Physiol Metab 292. Experiment Overall Design: Eight week old C57BL/6 mice were fed either chow (Labdiet 5008, Pharmserv) or KD (F3666, Bio-Serv) for six weeks. Livers were harvested in the morning in ad lib fed animals. Total RNA from 2-3 animals in each group was used for Affymetrix analysis.
transcription profiling by array, co-expression, growth condition
A high-fat, ketogenic diet induces a unique metabolic state in mice. Adam R Kennedy, Pavlos Pissios, Hasan Otu, Bingzhong Xue, Kenji Asakura, Noburu Furukawa, Frank E Marino, Fen-Fen Liu, Barbara B Kahn, Towia A Libermann, Eleftheria Maratos-Flier. Am J Physiol Endocrinol Metab 292(6):E1724-39 (2007)