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E-GEOD-76695 - Role of IL33 on muscle regneration in aging

Status
Released on 10 January 2016, last updated on 16 January 2016
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (5)
Description
We compare the effect of IL-33 vs PBS on muscle regneration from 22-month old mice 8 days post-injury. These analyses from whole muscle provide insight into the role of IL-33 on regeneration in the context of aging. The tibialis anterior muscle from male C57BL/6 mice (22 months old) were cryo-injured using a liquid nitrogen chilled metal probe for 8 seconds. Mice received 2 ug of rIL-33 via intraparitoneal injection the day prior and the day following cryoinjury. 8 days post-injury whole muscle was harvested and snap frozen in liquid nitrogen. Whole muscle was then homogenized directly in TriZol and gene expression profiling was performed on Affymetrix Mouse Gene 1.0 ST Arrays.
Experiment type
transcription profiling by array 
Contacts
CBDM Lab <cbdm@hms.harvard.edu>, Andrew Rhoads, Diane Mathis, Liang Yang, Wilson Kuswanto
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-76695.idf.txt
Sample and data relationshipE-GEOD-76695.sdrf.txt
Raw data (1)E-GEOD-76695.raw.1.zip
Processed data (1)E-GEOD-76695.processed.1.zip
Array designA-AFFY-130.adf.txt
Links