E-GEOD-766 - Transcription profiling of human RASF + IKB/Adtet + TNFa
Submitted on 21 October 2003, released on 26 September 2007, last updated on 10 June 2011
Rheumatoid arthritis synovial fibroblasts (RASF) were transected with an adenovirus (Ad) expressing a dominant negative (DN) form of Ik-B stimulated 18 hours later with TNF-a. Controls were RASF transfected with Adtet then treated with TNF-a 18 hours later. RNA was extracted 3 hours after TNF-a application. The same cell line was split into 6 lots grown up and then treated with the virus independent. Each of the Ik-B and Adtet has 3 replications. Data is Hu95Av2 processed with MAS 4.0.
transcription profiling by array, cell type comparison, co-expression