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E-GEOD-75202 - Cell specific response to IFNa

Status
Released on 20 November 2015, last updated on 29 November 2015
Organism
Mus musculus
Samples (52)
Array (1)
Protocols (5)
Description
We report cell specific responses to IFNa in 11 different peripheral immunocyte populations in the mouse. These cells represent the core ImmGen immunocyte lineage panel. Profiles from these were used to analyze cell specific responses to IFNa. In general a core set of ISG transcripts are induced in all cells. Smaller sets of ISGs were induced in a cell specific manner. In particular, splenic granulocytes and dendritic cells show restriced indcution of sets of ISGs. Male C57BL/6 mice were injected subcutaneously with 10,000U IFNa according to time course profile. Splenocytes/Peritoneal lavage suspensions were stained in multicolor antibody panels that were used to distinguish 11 different immunocyte populations (Immgen 11 cell set). These cells were sorted by FACS directly into TriZo and gene expression profiling was performed on Affymetrix Mouse Gene 1.0 ST Arrays.
Experiment type
transcription profiling by array 
Contacts
CBDM Lab <cbdm@hms.harvard.edu>, Adriana Ortiz-Lopez, Andrew Rhoads, Christophe Benoist, Devapregasan Moodley, Diane Mathis, Hideyuki Yoshida, Liang Yang, Sara Mostafavi
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-75202.idf.txt
Sample and data relationshipE-GEOD-75202.sdrf.txt
Raw data (2)E-GEOD-75202.raw.1.zip, E-GEOD-75202.raw.2.zip
Processed data (1)E-GEOD-75202.processed.1.zip
Array designA-AFFY-130.adf.txt
Links