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E-GEOD-75132 - TMEM45A, SERPINB5 and p16INK4A transcript levels are predictive for development of high-grade cervical lesions

Status
Released on 3 July 2016, last updated on 28 December 2016
Organism
Homo sapiens
Samples (41)
Array (1)
Protocols (5)
Description
Women persistently infected with human papillomavirus (HPV) type 16 are at high risk for development of cervical intraepithelial neoplasia grade 3 or cervical cancer (CIN3+). We aimed to identify biomarkers for progression to CIN3+ in women with persistent HPV16 infection. In this prospective study, 11,088 women aged 20–29 years were enrolled during 1991-1993, and re-invited for a second visit two years later. Cervical cytology samples obtained at both visits were tested for HPV DNA by Hybrid Capture 2 (HC2), and HC2-positive samples were genotyped by INNO-LiPA. The cohort was followed for up to 19 years via a national pathology register. To identify markers for progression to CIN3+, we performed microarray analysis on RNA extracted from cervical swabs of 30 women with persistent HPV16-infection and 11 HPV-negative women. After further validation, we found that high mRNA expression levels of TMEM45A, SERPINB5 and p16INK4a were associated with increased risk of CIN3+ in persistently HPV16-infected women. We aimed at identifying genes differentially expressed in women with persistent HPV16 infection that either progressed to CIN3+ or not. As a test of principle we first compared HPV16 persistently infected women with HPV-negative women.
Experiment type
transcription profiling by array 
Contacts
Frank Stubenrauch <frank.stubenrauch@med.uni-tuebingen.de>, Anna Manawapat-Klopfer, Hans Gmuender, Rainer Russ, Susanne K Kjaer, Thomas Iftner
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-75132.idf.txt
Sample and data relationshipE-GEOD-75132.sdrf.txt
Raw data (2)E-GEOD-75132.raw.1.zip, E-GEOD-75132.raw.2.zip
Processed data (1)E-GEOD-75132.processed.1.zip
Array designA-AFFY-44.adf.txt
Links