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E-GEOD-74745 - Expression data from brown planthhoper (BPH) infested and non-infested rice

Status
Released on 6 November 2015, last updated on 1 December 2015
Organism
Oryza sativa
Samples (12)
Array (1)
Protocols (7)
Description
KMD is genetically engenered to be highly resistant to lepidopteran pests through expressing a synthetic cry1Ab gene and its parent non-transgenic rice is Xiushui 11.The developmental duration of BPH feeding on KMD2 was significantly delayed. And moreover, the fecundity of BPH was significantly lower when fed on Bt rice than on the non-Bt parental plants.To investigate unintended effects in KMD2 that causes changes in BPH performance, we performed microarray (GeneChip) analysis to compare the gene expression profiles between Bt rice and non-transgenic parental plants in response to BPH infestation. We used microarrays to detect Bt-independent variation, which might render Bt rice more defensive or less nutritious to BPH. For BPH treatment, 10 second-instar nymphs were infested onto each 30-day-old seedling. After 72 h, the BPH nymphs were carefully removed and rice shoots of both BPH-infested and non-infested plants were sampled for microarray analysis. There were four treatments: Xiushui 11-non infested, Xiushui 11-BPH infested, KMD2-non infested, KMD2-BPH infested, three biological replications.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-74745.idf.txt
Sample and data relationshipE-GEOD-74745.sdrf.txt
Raw data (1)E-GEOD-74745.raw.1.zip
Processed data (1)E-GEOD-74745.processed.1.zip
Array designA-AFFY-126.adf.txt
Links