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E-GEOD-74463 - Altered lipid metabolism in the aging kidney identified by three layered omic analysis

Status
Released on 30 October 2015, last updated on 8 December 2015
Organism
Mus musculus
Samples (9)
Array (1)
Protocols (7)
Description
Aging-related diseases and their comorbidities affect the life quality of a constantly growing proportion of our population. Age-associated changes of kidney structure and function are considerable contributors to the dramatically increased incidence of chronic kidney disease world-wide which has been identified to be a prominent cardiovascular risk factor. In order to detect molecular mechanisms involved in kidney aging we analyzed gene expression profiles of kidneys from adult and aged wild-type mice by a three-layered omics strategy. To this end, transcriptomic, proteomic and targeted lipidomic profiles of young and aged mice were generated and integrated. Transcriptome and proteome analyses revealed differential expression of genes involved primarily in lipid metabolism and immune response. Additional lipidomic analyses uncovered significant age-related differences in the total amount of phosphatidylethanolamines, phosphatidylcholines and sphingomyelins as well as in subspecies of phosphatidylserines and ceramides, while total ceramide levels remained unchanged. By integration of these datasets we identified Aldh1a1, a key enzyme in vitamin A metabolism specifically expressed in the medullary ascending limb, as one of the most prominent upregulated proteins in old kidneys. Moreover, ceramidase Asah1 was highly expressed in aged kidneys, consistent with a decrease in ceramide C16. In summary, our data suggest that changes in lipid metabolism are involved in the process of kidney aging and in the development of chronic kidney disease. 14 weeks females (4 replicates); 96 weeks females (5 replicates)
Experiment type
transcription profiling by array 
Contacts
Christine Elisabeth Kurschat <christine.kurschat@uk-koeln.de>, Bernard Schermer, Bianca Habermann, Björn Schumacher, Christine E Kurschat, Fabian Braun, Jan H Hoeijmakers, Markus M Rinschen, Martijn E Dollé, Peter Frommolt, Roman-Ulrich Müller, Thomas Benzing, Valerie Bartels
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-74463.idf.txt
Sample and data relationshipE-GEOD-74463.sdrf.txt
Raw data (1)E-GEOD-74463.raw.1.zip
Processed data (1)E-GEOD-74463.processed.1.zip
Array designA-AFFY-130.adf.txt
Links