Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-74459 - Translation State Array Assay for C elegans IFE-1-dependent mRNAs

Status
Released on 7 January 2016, last updated on 17 January 2016
Organism
Caenorhabditis elegans
Samples (12)
Array (1)
Protocols (7)
Description
Relative polysomal loading changes for wild type (N2) versus ife-1(bn127) C. elegans strains Strain ife-1(bn127) is null for the gene encoding one of five eIF4E isoforms in the worm, IFE-1. Only this isoform associates with P granules. Resolution of translating and non-translating mRNAs from WT and mutant worms on 10-45% sucrose gradients. Comparision was by ratios of polysomal content. Please note that each sample data table contains raw signal directly imported from the Affymetrix files and the ratio of signals, P/(NP+P), and fold-changes were further calculated. The TSAA_overview.xls contains more datails about the TSAA procedure and 'complete_data.xlsx' contains complete TSAA data output.
Experiment type
transcription profiling by array 
Contacts
Andrew J Friday, Brett D Keiper, J K Morrison, Jenna L Hoffman, Melissa A Henderson
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-74459.idf.txt
Sample and data relationshipE-GEOD-74459.sdrf.txt
Raw data (1)E-GEOD-74459.raw.1.zip
Processed data (1)E-GEOD-74459.processed.1.zip
Additional data (1)E-GEOD-74459.additional.1.zip
Array designA-AFFY-60.adf.txt
Links