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E-GEOD-74279 - Positional proteomics reveals differences in N-terminal proteoform stability

Status
Released on 23 October 2015, last updated on 8 December 2015
Organism
Homo sapiens
Samples (2)
Protocols (3)
Description
To understand the impact of alternative translation initiation on a proteome, we performed the first study on protein turnover using positional proteomics and ribosome profiling to distinguish between N-terminal proteoforms of individual genes. Overall, we monitored the stability of 1,941 human N-terminal proteoforms, including 147 N-terminal proteoform pairs that originate from alternative translation initiation, alternative splicing or incomplete processing of the initiator methionine. Ribosome profiling of lactimidomycin and cycloheximide treated human Jurkat T-lymphocytes
Experiment types
other, RNA-seq of coding RNA 
Contacts
Daria Gawron <daria.gawron@ugent.be>, Elvis Ndah, Kris Gevaert, Petra Van Damme
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-74279.idf.txt
Sample and data relationshipE-GEOD-74279.sdrf.txt
Processed data (2)E-GEOD-74279.processed.1.zip, E-GEOD-74279.processed.2.zip
Links