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E-GEOD-74151 - Expression data from three types of spermatogonial stem cells

Status
Released on 30 October 2015, last updated on 13 December 2015
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
Multipotent spermatogonial stem cells (mSSCs) derived from SSCs are a potential new source of individualized pluripotent cells in regenerate medicine such as ESCs. We hypothesized that the culture-induced reprogramming of SSCs was mediated by a mechanism different from that of iPS, and was due to up-regulation of specific pluripotency-related genes during cultivation. Through a comparative analysis of expression profile data, we try to find cell reprogramming candidate factors from mouse spermatogonial stem cells. We used microarrays to analyze the gene expression profiles of culture-induced reprogramming converting unipotent spermatogonial stem cells to pluripotent spermatogonial stem cells. Three types of spermatogonial stem cells were mechanically collected according to morphological criteria for RNA extraction and hybridization on Affymetrix microarrays.
Experiment type
transcription profiling by array 
Contacts
Dong R Lee, Insuk Lee
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-74151.idf.txt
Sample and data relationshipE-GEOD-74151.sdrf.txt
Raw data (1)E-GEOD-74151.raw.1.zip
Processed data (1)E-GEOD-74151.processed.1.zip
Array designA-AFFY-45.adf.txt
Links