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E-GEOD-74126 - Rapid and affordable genome-wide bisulfite DNA sequencing by XmaI-RRBS

Status
Released on 1 November 2015, last updated on 3 December 2015
Organism
Homo sapiens
Samples (7)
Protocols (2)
Description
We describe XmaI-RRBS method for rapid and affordable genome-wide DNA methylation analysis, with library preparation taking only four days and sequencing possible within four hours. Small sizes of the XmaI-RRBS libraries allow their multiplexing and sequencing on the benchtop high-throughput machines. Described here is the first RRBS protocol validated for the Ion Torrent Personal Genome Machine. DNA from MCF7 cell line and 6 normal breast samples (total 7 samples) were subjected to reduced representation bisulfite sequencing analysis (XmaI-RRBS) by using Ion Torrent platform.
Experiment type
methylation profiling by high throughput sequencing 
Contacts
Alexander S. Tanas <geo@ncbi.nlm.nih.gov>, Alexander S Tanas, Vladimir V Strelnikov
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-74126.idf.txt
Sample and data relationshipE-GEOD-74126.sdrf.txt
Processed data (1)E-GEOD-74126.processed.1.zip
Links