E-GEOD-7412 - Transcription profiling of mouse IgM+IgD+ cells and srf-deficient IgM+IgD+ cells isolated from spleens to investigate the role of serum response factor

Status
Submitted on 30 March 2007, released on 15 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (5)
Description
Serum response factor (SRF), a MADS-box transcription factor, is essential for murine embryonic development and for the function of muscle cells and neurons. SRF and its transcriptional co-factors are broadly expressed. To determine the in vivo role of SRF in developing lymphocytes we specifically inactivated the murine Srf gene during T or B cell development using lymphocyte-specific Cre transgenic mouse lines. T cell-specific Srf deletion led to a severe block in thymocyte development at the transition from double to single positive stage. The few residual T cells detectable in the periphery retained at least one functional Srf allele, thereby demonstrating the importance of SRF in T cell development. In contrast, deletion of Srf in developing B cells did not interfere with the growth and survival of B cells in general, yet led to a complete loss of marginal zone B cells and a marked reduction of the CD5+ B cell subset. Our study also revealed a contribution of SRF to the expression of the surface molecules IgM, CD19, and the chemokine receptor 4 in B lymphocytes. Experiment Overall Design: Comparison of the gene expression profile between murine wildtype IgM+IgD+ cells and srf-deficient IgM+IgD+ cells isolated from spleens
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Serum response factor contributes selectively to lymphocyte development. Anne Fleige, Siegfried Alberti, Lothar Gröbe, Ursula Frischmann, Robert Geffers, Werner Müller, Alfred Nordheim, Angela Schippers. J Biol Chem 282(33):24320-8 (2007)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-7412.idf.txt
Sample and data relationshipE-GEOD-7412.sdrf.txt
Raw data (1)E-GEOD-7412.raw.1.zip
Processed data (1)E-GEOD-7412.processed.1.zip
Array designA-AFFY-36.adf.txt
R ExpressionSetE-GEOD-7412.eSet.r
Links